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叶应旺
发布时间:2018-09-11    阅读次数: 2605

姓 名:
叶应旺
职 称:
教授
职 务:
 
所属系:
食品安全系
邮 箱:
yeyingwang04@126.com
电 话:
 
个人学习工作简介
1997年9月---2001年7月:  广东海洋大学 水产学院 水产养殖专业
2004年9月---2009年7月:  中国科学院南海海洋研究所 海洋生物学专业
主要研究领域与方向
1.食源性致病微生物检测与控制研究
2.食源性致病微生物群体生物学研究
3.食源性致病微生物致病机理及肠道微生态学研究
开设课程
食品法规与品质控制、食品标准与法规、食品微生物检验学、食品安全与检测技术(研究生课程)
近年的科研项目(五年内)
1、国家自然科学基金面上项目,31671951,阪崎克罗诺杆菌鞭毛介导的运动性及生物膜形成的微环境响应及LuxS调控研究,2017/01-2020/12,直接经费65万元。
2、国家自然科学基金青年项目,31201292,基于抑制消减杂交和比较蛋白质组学技术发掘阪崎肠杆菌毒力相关因子的研究,2013/01-2015/12,23万元,已结题。
3、安徽省重大科技专项,15czz03109,婴幼儿配方奶粉中风险因子监测体系构建及品质控制关键技术提升研究,2015/10-2017/12,100万元,到校经费40万元,在研
4、广州市协同创新重大专项,201604020036,基于纳米增敏效应的食源性致病菌快速检测技术研究。2016/04-2019/3,100万元,到校经费35万元,在研
5、广东省中国科学院全面战略合作重点项目,2012B090400017,重要食源性致病菌多分子靶标与荧光定量检测技术研究,2012/10-2015/09,150万元,到校经费52.5万元,已结题。
6、安徽省自然科学基金项目,1208085QC64,奶粉源阪崎肠杆菌毒力基因发掘及毒力差异与DNA指纹图谱关联性研究,2012/07-2014/06,4万元,已结题
7、广东省中国科学院全面战略合作项目,2011B0903777,阪崎肠杆菌种间特异性靶标的发掘及分子检测与分型技术研究,2011/10-2013/9,30万元,到校经费12万元,已结题。
8、广东省科技计划项目,2011B020314001,副溶血弧菌免疫捕获与环介导等温扩增快速检测技术研究,2011/10-2013/2013/09,8万元,到校经费2.4万元,已结题。

二、参加的科研项目
1.国家自然科学基金面上项目,华东地区粒毛盘菌资源与生物活性物质研究、2011/01-2013/12、32万元、已结题、第三。
2、国家自然科学基金面上项目,基于分子修饰的菜籽多糖对肠道益生菌的增值作用及构效关系研究、2012/01-2015/12、60万元、已结题、第三。
著作论文(代表作)
(1) Yingwang Ye, Ling Na, Han Yongjia, Qingping Wu. Detection and prevalence of pathogenic Yersinia enterocolitica in refrigerated and frozen dairy products by duplex PCR and dot hybridization targeting the virF and ail genes. Journal of Dairy Science. 2014, 97: 6785-6791.
(2)Rui Jiao, Jina Gao, Xiyan Zhang, Maofeng Zhang, Qingping Wu, Jumei Zhang, Yingwang Ye. Effect of High pressure processing on the inactivation of C. sakazakii in whole milk and skim milk. Journal of Dairy Science. 2016, 99: 7881-7885.
(3) Yingwang Ye, Qingping Wu, Xiaoke Xu, Xiaojuan Yang, Xiaohui Dong, Jumei Zhang. The phenotypic and genotypic Characterization of Enterobacter sakazakii isolates from Infant Formula Milk,Journal of Dairy Science, 2010, 93: 2315-2320.
(4) Yingwang Ye, Hui Li, Na Ling, Yongjia Han, Qingping Wu, Xiaoke Xu, Rui Jiao, Jina Gao. Identification of potential virulence factors of Cronobacter sakazakii isolates by comparative proteomic analysis. International Journal of Food Microbiology, 2016, 217: 182-188.
(5) Yingwang Ye, Qingping Wu, Lin Yao, Xiaohui Dong, Kui Wu, Jumei Zhang. Analysis of a consensus fragment in ERIC-PCR fingerprinting of Enterobacter sakazakii. International Journal of Food Microbiolology. 2009, 132: 172-175.
(6) Yingwang Ye, Rui Jiao, Jina Gao, Hui Li, Na Ling, Qingping Wu, Jumei Zhang, Xiaoke Xu. Proteins involved in responses to biofilm and planktonic modes in Cronobacter sakazakii. LWT - Food Science and Technology. 2016. 65: 1093-1099.
(7) Yingwang Ye, Na Ling, Rui Jiao, Qinpging Wu, Yongjia Han, Jina Gao. Effects of culture conditions on the biofilm formation of Cronobacter sakazakii strains and distribution of genes involved in biofilm formation. LWT-Food Science and Technology. 2015, 61:1-6.
(8) Qingping Wu, Yingwang Ye, Fei Li, Jumei Zhang, Weipeng Guo. Prevalence and genetic characterization of Pseudomonas aeruginosa in drinking water in Guangdong Province of China. LWT - Food Science and Technology, 2016, 69: 24-31.
(9) Hui Li, Yingwang Ye, Na Ling, Qingping Wu, Jumei Zhang. Inhibitory effects of D-tryptophan on biofilm development by the foodborne Cronobacter sakazakii. International Dairy Journal, 2015, 49: 125-129.
(10) Yingwang Ye, Hui Li, Qingping Wu, Mengshi Chen, Yudong Lu, Chunming Yan. Isolation and Phenotypic Characterization of Cronobacter from Dried Edible Macrofungi Samples. Journal of Food Science, 2014, 79: 1382-1386.
(11) Yingwang Ye, Qingping Wu, Jumei Zhang, He Jiang, Wang Hu. Detection of Viable Cronobacter spp. (Enterobacter sakazakii) by One-Step RT-PCR in Dry Aquatic Product. Journal of Food Science, 2012, 77: M616-M619.
(12)Yingwang Ye, Hui Li, Qingping Wu, Na Ling, Yongjia Han. Immunoca ptured-Loop Mediated Isothermal Amplication assay for detection of Vibrio Parahaemolyticus in seafood. Journal of Food Safety. 2014, 34: 21-25.
(13) Yingwang Ye, Jina Gao, Rui Jiao, Hui Li, Qingping Wu, Jumei Zhang, Xian Zhong. The Membrane Proteins Involved in Virulence of Cronobacter sakazakii Virulent G362 and Attenuated L3101 Isolates. Frontiers in Microbiology. 2015, 6: article 1238.
(14) Yingwang Ye, Na Ling, Yongjia Han, Xiaodong Cao, Qingping Wu. Detection of Cronobacter on gluB and differentation of four Cronobacter speices by RFLP-PCR typing. Journal of Food Safety. 2015, 35: 422-427.
(15) Yingwang Ye, Na Ling, Rui Jiao, Qingping Wu, Yongjia Han, Jina Gao. Effects of Ca2+ and Mg2+ on the biofilm formation of Cronobacter sakazakii strains from powdered infant formula. Journal of Food Safety, 2015, 35: 416-421.
(16) Yingwang Ye, Li Hui, Wu Qingping, Zhang Jumei, Lu Yudong. The Cronobacter sp. in milk and dairy products: Detection and typing. International Journal of Dairy Technology, 2014, 67: 167-175.
(17) Yingwang Ye, Yelin Jiang, Tengfei Fan, Qihuang Jiang, Yunsheng Cheng, Jianfeng Lu, Lin Lin. Resistance Characterization, Virulence Factors, and ERIC-PCR Fingerprinting of Aeromonas veronii Strains Isolated from Diseased Trionyx sinensis,Foodborne Pathogens and Disease,2012,9(11):1053-1055.
(18) Yingwang Ye,Qihuan Jiang, Qingping Wu, Jumei Zhang, Jianfeng Lu, Lin Lin. The characterization and comparison of Staphylococcus aureus by antibiotic susceptibility testing, enterobacterial repetitive intergenic consensus-polymerase chain reaction, and random amplified polymorphic DNA-polymerase chain reaction. Foodborne Pathogens and Disease. 2012, 9: 168-171.
(19) Yingwang Ye, Qingping Wu, Jumei Zhang, Jianfeng Lu, Lin Lin. Isolation of Salmonella from Meat Samples and Characterization by Enterobacterial Repetitive Intergenic Consensus-Polymerase Chain Reaction and Antibiotics Test. Foodborne Pathogens and Disease. 2011, 8: 935-937.
(20) Yingwang Ye, Qingping Wu, Jumei Zhang, Jianfeng Lu, Lin Lin, Detection of Cronobacter (Enterobacter sakazakii)in infant formula and phylogenetic analysis on a-glucosidase genes. Journal of Food Safety. 2011, 31: 185-189.
(21) Yingwang Ye, Qingping Wu, Lin Yao, Xiaohui Dong, Kui Wu, Jumei Zhang. A comparison of Polymerase Chain Reaction and International Organization for Standardization Methods for Determination of Enterobacter sakazakii Contamination of Infant Formulas from Chinese Mainland Markets,Foodborne Pathogens and Disease. 2009, 6: 1229-1234.
(22) Yingwang Ye, Qingping Wu, Yanhong Zhou, Xiaohui Dong, Jumei Zhang. Analysis of major band of Enterobacter sakazakii by ERIC-PCR and development of a species-specific PCR for detection of Ent. sakazakii in dry food samples. Journal of Microbiological Methods. 2008, 75: 392-397.
(23) Na Ling, Yingwang Ye, Hui Li, Qingping Wu, Yongjia Han, Yudong Lu. A novel procedure on vancomycin, cephalotin, and sucrose for isolation of Cronobacter spp. from powdered infant formula. Journal of Food Safety. 2015, 35: 257-262.
(24) Youhao Zhong, Yinji Chen, Li Yao, Diping Zhao, Lei Zheng, Guodong Liu, Yingwang Ye, Wei Chen. Gold nanoparticles based lateral flow immunoassay with largely amplified sensitivity for rapid melamine screening. Microchim Acta, 2016, 183:189-194.
(25)Rui Jiao, Jina Gao, Xiyan Zhang, Maofeng Zhang, Jiren Chen, Qingping Wu, Jumei Zhang, Yingwang Ye(通讯作者). Effects of vacuum freeze drying on inactivation of Cronobacter sakazakii ATCC29544 in liquid media with different initial inoculum levels. Journal of Dairy Science. 2017,100: 1674-1678.
(26) Xiaodong Cao, Jiaona Gao, Yingwang Ye, Pengfei Wang, Shun Ding, Yongkang Ye, Hanju Sun, Electroanalysis, DOI: 10.1002/elan.201500508.
(27) Yongkang Ye, Shun Ding, Yingwang Ye, Houchuan Xu, Xiaodong Cao, Sheng Liu and Hanju Sun, Microchimca Acta, 2015, 9-10, 1783-1789.
(28) Xiaoke Xu,Qingping Wu,Jumei Zhang,Yingwang Ye,Xiaojuan Yang,Xiaohui Dong,Occurrence and Characterization of Cronobacter spp.in Powdered Formula from Chinese Retail Markets,Foodborne Pathogens and Disease, 2014, 11: 307-312.
(29) Lin Lin, Shun Lv, Xiaorong Zhang, Jianfeng Lu, Yingwang Ye, Shaotong Jiang. ACE Inhibitory Peptides Derived from Aquatic Protein. CURRENT PROTEIN & PEPTIDE SCIENCE. 2013, 14: 235-241.
(30) Ming Ye, Yan Wang, Gengyi Guo, Yunlong He, Ying Lu, Yingwang Ye, Qinghua Yang, Peizhou Yang. Physicochemical characteristics and antioxidant activity of arginine-modified melanin from Lachnum YM-346. Food Chemistry, 2012, 135: 2490-2497.
(31) Ming Ye, Wuxi Chen, Tao Qiu, Ruyue Yuan, Yingwang Ye, Jingmin Cai. Structural characterisation and anti-ageing activity of extracellular polysaccharide from a strain of Lachnum sp. Food Chemistry, 2012, 132: 338-343.
(32) Ming Ye, Tao Qiu, Wei Peng, Wuxi Chen, Yingwang Ye, Yingren Lin. Purification, characterization and hypoglycemic activity of extracellular polysaccharides from Lachnum calyculiforme. Carbohydrate Polymers, 2011, 86: 285-290.
(33) Yanhong Zhou, Qingping Wu, Xiaoke Xu, Xiaojuan Yang, Yingwang Ye, Jumei Zhang. Development of an immobilization and detection method of Enterobacter sakazakii from powdered infant formula. Food Microbiology, 2008, 25: 648-652.
(34) 吴清平,董晓晖,张菊梅,叶应旺,徐晓可,杨小鹃,吴葵. 阪崎肠杆菌分类与致病机制. 微生物学报,2010,50(7):841-846.
(35) 吴清平,吴葵,叶应旺,董晓晖,张菊梅.群体感性及其在动物病原菌致病中的作用. 微生物学报,2009,49(7):853-858.
(36) 吴清平 ,叶应旺,郭伟鹏,杨宁. 阪崎肠杆菌生物学特性及检测技术. 微生物学通报,2006,33(6):99-103.

专利
(1)叶应旺,李辉,凌娜、韩永佳.用于克罗诺杆菌冷热损伤修复的前增菌培养基。专利号:ZL201410080751.9.
(2)叶应旺,裴陆送、吴向俊、韩永佳、凌娜。茶香风味封鳊鱼加工制作工艺。专利号:ZL201410141870.0.
(3)叶应旺,凌娜、韩永佳。一种区分克罗诺杆菌属不同种的PCR-RFLP分子分型方法。ZL201410770092.1.
获奖情况
(1)国家食品领域知名期刊Food Research International(JCR2区) 编委
(2)合肥市首届食品药品应急管理专家委员会委员(11人)
(3)中华鳖生态养殖关键即使创制与集成应用。负责疾病病原分离与鉴定及分子分型工作。安徽省科技进步三等奖(R6)
(4)中国科学院冠名奖学金“地奥奖”二等奖。
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